As an essential tool for quantitative analysis in molecular biology, the real-time PCR system is extensively utilized across various fields, including scientific research, clinical diagnostics, quality and safety testing, and forensic analysis.
Key Features:
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Equipped with up to six fluorescence detection channels for efficient multiplex PCR.
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Minimizes multi-color crosstalk and edge effects, eliminating the need for ROX correction.
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Advanced optical scanning detection system ensures high precision and stability.
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Incorporates innovative scanning methods and time-resolved signal separation technology for enhanced accuracy.
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Features a unique edge temperature compensation technology for consistent results.
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Supported by user-friendly software designed for intuitive operation and data management.
| Channel 6 | Channel 5 | Channel 4 |
| NED/Cy3/TAMRA
543±20/584±20 |
ROX/Texas Red
571±20/610±20 |
FAM/SYBR
480±10 |
| VIC/HEXTETJOE
532±10/564±20 |
CY5/Quasar 670
640±20/692±40 |
/518±10FAM/SYBR
480±10/518±10 |
| Channel 3 | Channel 2 | Channel 1 |
| Temperature control system | |
| Sample capacity | 0.1ml PCR tubes×96, 8×12 PCR plate or 96 well plate ×1 |
| Reaction volume | 10 – 50 μl |
| Thermal cycle technology | Peltier |
| Max. Heating/Cooling rate | 6.0°C/s |
| Heating temperature range | 4 – 100°C |
| Temperature accuracy | ± 0.2°C |
| Temperature uniformity | ±0.2°C @60°C,
±0.2°C @95°C |
| Temperature gradient setting range | 30 – 100°C |
| Temperature gradient difference
setting range |
1 – 36°C |
| Detection system | |
| Excitation light source | 5/6 monochrome high efficiency LEDs |
| Detection device | PMT |
| Detection mode | Time-resolved signal separating technology |
| Excitation/detection
wavelength range |
455 – 650nm/510 – 715nm |
| Fluorescent channels | 4/6 channels |
| Supported dye | FAM/SYBR Green, VIC/HEX ROX,Cy5,Cy3(only for x6) |
| Sensitivity | Single copy gene |
| Resolution | 1.33 folds copy number difference can be distinguished in single-
plex qPCR |
| Dynamic range | 10 orders of magnitude copies |
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